Bacterial cell culture media Staphylococcus epidermidis

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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Protocol tips
For the detection of biofilm formation, S. epidermidis was cultured in TSA medium supplemented with 0.5% glucose. When appropriate, antibiotics were used at the following concentrations: erythromycin (5 μg/ml), spectinomycin (100 μg/ml), chloramphenicol (10 μg/ml), ampicillin (100 μg/ml), and kanamycin (50 μg/ml).

Thermo Fisher Scientific

Protocol tips
The test tube of 28- day old static cultures (MH strain) was vigorously vortexed for 30 sec, 50 mL were spot deposited on each filter, spread with a spatula and incubated for one day at 37 degrees C
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