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|For the detection of biofilm formation, S. epidermidis was cultured in TSA medium supplemented with 0.5% glucose. When appropriate, antibiotics were used at the following concentrations: erythromycin (5 μg/ml), spectinomycin (100 μg/ml), chloramphenicol (10 μg/ml), ampicillin (100 μg/ml), and kanamycin (50 μg/ml).
|The test tube of 28- day old static cultures (MH strain) was vigorously vortexed for 30 sec, 50 mL were spot deposited on each filter, spread with a spatula and incubated for one day at 37 degrees C
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