cDNA synthesis Bacteria -
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5 years ago
5 years ago by
Stefan Fuhrmann
Will presence of EDTA effect cDNA synthesis
I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?
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