Cell Culture Contamination Detection Kit Fungi

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 4 matching solutions for this experiment

Fungi-Fluor® Kit for Fungal Detection

Polysciences

Upstream tips	Protocol tips	Downstream tips
• Kit may be used for the rapid identification of various fungal infections in fresh or frozen clinical specimens, paraffin or glycol methacrylate embedded tissues. • Typical specimens include sputum, bronchoalveolar lavage (BAL), bronchial wash, skin scrapings and tissue biopsies.	• Fungi-Fluor Staining Solution is a 0.05% solution of high purity Cellufluor, an active ingredient of Calcofluor-white fluorescent stain, in deionized water with potassium hydroxide added as a clearing agent. • Fungi-Fluor Counterstaining Solution B is an aqueous solution of Evans Blue dye, which is used to reduce background fluorescence.

Upstream tips
• Kit may be used for the rapid identification of various fungal infections in fresh or frozen clinical specimens, paraffin or glycol methacrylate embedded tissues. • Typical specimens include sputum, bronchoalveolar lavage (BAL), bronchial wash, skin scrapings and tissue biopsies.
Protocol tips
• Fungi-Fluor Staining Solution is a 0.05% solution of high purity Cellufluor, an active ingredient of Calcofluor-white fluorescent stain, in deionized water with potassium hydroxide added as a clearing agent. • Fungi-Fluor Counterstaining Solution B is an aqueous solution of Evans Blue dye, which is used to reduce background fluorescence.

Manufacturer protocol 1 Relevant paper

MicroSEQ™ D2 rDNA Fungal PCR Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
• This easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. • To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.	• Detection Method: Gel Electrophoresis • Sample Type: DNA • Technique: SBT • Libraries: Fungal Library (> 1100 species), Customizable

Upstream tips
• This easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. • To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.
Protocol tips
• Detection Method: Gel Electrophoresis • Sample Type: DNA • Technique: SBT • Libraries: Fungal Library (> 1100 species), Customizable

Manufacturer protocol

Fast MicroSEQ™ D2 Fungal rDNA PCR Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
• The sequencing component of the kit is required for species identification. • Kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens	• Detection Method: Gel Electrophoresis • GC-Rich PCR Performance: Low • Technique: SBT • Libraries: Fungal Library (> 1100 species), Customizable

Upstream tips
• The sequencing component of the kit is required for species identification. • Kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens
Protocol tips
• Detection Method: Gel Electrophoresis • GC-Rich PCR Performance: Low • Technique: SBT • Libraries: Fungal Library (> 1100 species), Customizable

Manufacturer protocol

Cell Culture Contamination Detection Kit

Invivogen

Upstream tips	Protocol tips	Downstream tips
• Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram-negative bacteria by slide preparation.	• Detection Method: Fluorescent • The kit provides sufficient material to perform approximately 200 contamination assays

Upstream tips
• Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram-negative bacteria by slide preparation.
Protocol tips
• Detection Method: Fluorescent • The kit provides sufficient material to perform approximately 200 contamination assays

Manufacturer protocol

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox