Cell Culture Contamination Detection Kit Yeast

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

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Found 6 matching solutions for this experiment

AMPIPROBE® Candida 1 Assay Kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
• The nucleotide sequences of the PCR primers have been optimized to target sequences within species-specific regions of the Candida genome. • Target detection is based on fluorescence decay when successive rounds of amplification bring fluorophore and quencher-labeled primer pairs in close proximity resulting in FRET.	• Sensitivity: Candida albicans 98.20% Candida glabrata - 97.10% Candida krusei - 100.00% • Loss of fluorescence below a defined threshold in a particular channel indicates sample positivity for the corresponding Candida species.

Upstream tips
• The nucleotide sequences of the PCR primers have been optimized to target sequences within species-specific regions of the Candida genome. • Target detection is based on fluorescence decay when successive rounds of amplification bring fluorophore and quencher-labeled primer pairs in close proximity resulting in FRET.
Protocol tips
• Sensitivity: Candida albicans 98.20% Candida glabrata - 97.10% Candida krusei - 100.00% • Loss of fluorescence below a defined threshold in a particular channel indicates sample positivity for the corresponding Candida species.

Manufacturer protocol

Remel™ Candida albicans Test Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
• Rapid test for the detection of enzymes L-proline aminopeptidase and Β-galactosaminidase in yeast cells for the presumptive identification of Candida albicans cultured on laboratory media. • A rapid and sensitive method utilizing a combination of p-nitrophenyl-N-acetyl-Β-D-galactosaminide (NGL) and L-proline-Β-naphthylamide (PRO)	• Includes: 50 test discs; 1 × 45mL vial 03% sodium hydroxide; 1 vial color developer; 50 plastic tubes with caps; wooden applicator sticks.

Upstream tips
• Rapid test for the detection of enzymes L-proline aminopeptidase and Β-galactosaminidase in yeast cells for the presumptive identification of Candida albicans cultured on laboratory media. • A rapid and sensitive method utilizing a combination of p-nitrophenyl-N-acetyl-Β-D-galactosaminide (NGL) and L-proline-Β-naphthylamide (PRO)
Protocol tips
• Includes: 50 test discs; 1 × 45mL vial 03% sodium hydroxide; 1 vial color developer; 50 plastic tubes with caps; wooden applicator sticks.

Manufacturer protocol

Human Candida Albicans ELISA Kit

MyBioSource.com

Upstream tips	Protocol tips	Downstream tips
• Kit for analyzing the presence of the Candida Albicans, ELISA Kit target analytes in biological samples. • The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Candida Albicans.	• Samples: Human serum, plasma or cell culture supernatant • Assay Type: Quantitative Sandwich • Detection Range: 100 ng/mL-1.56 ng/mL • Sensitivity: Up to 0.5 ng/mL

Upstream tips
• Kit for analyzing the presence of the Candida Albicans, ELISA Kit target analytes in biological samples. • The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Candida Albicans.
Protocol tips
• Samples: Human serum, plasma or cell culture supernatant • Assay Type: Quantitative Sandwich • Detection Range: 100 ng/mL-1.56 ng/mL • Sensitivity: Up to 0.5 ng/mL

Manufacturer protocol

Candida albicans Detection Kits (Cat. TM34000, TM34010, EP34000)

Norgen Biotek

Upstream tips	Protocol tips	Downstream tips
There are 2 kits available for the detection of Candida albicans: • Candida albicans TaqMan PCR/RT-PCR Kit, 24 reactions • Candida albicans End-Point PCR/RT-PCR Kit, 24 reactions	• Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay.

Upstream tips
There are 2 kits available for the detection of Candida albicans: • Candida albicans TaqMan PCR/RT-PCR Kit, 24 reactions • Candida albicans End-Point PCR/RT-PCR Kit, 24 reactions
Protocol tips
• Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay.

Manufacturer protocol

Cell Culture Contamination Detection Kit

Invivogen

Upstream tips	Protocol tips	Downstream tips
• Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram-negative bacteria by slide preparation.	• Detection Method: Fluorescent • The kit provides sufficient material to perform approximately 200 contamination assays

Upstream tips
• Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram-negative bacteria by slide preparation.
Protocol tips
• Detection Method: Fluorescent • The kit provides sufficient material to perform approximately 200 contamination assays

Manufacturer protocol

Candida albicans antigen ELISA Kit

Creative Diagnostic

Upstream tips	Protocol tips	Downstream tips
• Candida albicans antigen ELISA Kit is a quantitative and qualitative immunoassay for the detection of Candida antigen in human serum or plasma. The assay is recommended for the detection of systemic candidosis.

Upstream tips
• Candida albicans antigen ELISA Kit is a quantitative and qualitative immunoassay for the detection of Candida antigen in human serum or plasma. The assay is recommended for the detection of systemic candidosis.

Manufacturer protocol

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