Cell cycle assay human - A2780

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

BD Cycletest™ Plus DNA Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- After cells were seeded, they were treated with control or SP-6-27 treated (0.5 uM, 24h)	- PI staining was performed for 10 min at 4°C

Upstream tips
- After cells were seeded, they were treated with control or SP-6-27 treated (0.5 uM, 24h)
Protocol tips
- PI staining was performed for 10 min at 4°C

Manufacturer protocol Publication protocol 1 Relevant paper

FxCycle™ Far Red Stain

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were incubated for 60 min in the presence of 10 μM 5-ethynyl-2’-deoxyuridine (EdU) at 37°C.

Protocol tips
- Cells were incubated for 60 min in the presence of 10 μM 5-ethynyl-2’-deoxyuridine (EdU) at 37°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
- Cells were analysed for cell cycle after 72h cell culture

Upstream tips
- Cells were analysed for cell cycle after 72h cell culture

Manufacturer protocol Publication protocol 1 Relevant paper

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