Cell cycle assay human - A549

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 2 matching solutions for this experiment

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Cells are fixed with 70% cold ethanol, and stored at −20 °C for 1 h - Cells were washed with PBS containing 0.1% triton X-100 on a shaking water bath at 37 °C, 40 rpm for 30 min.	- Store tubes at 4°C protected from light prior to analysing. Analyze on the flow cytometer within 1 hour.

Protocol tips
- Cells are fixed with 70% cold ethanol, and stored at −20 °C for 1 h - Cells were washed with PBS containing 0.1% triton X-100 on a shaking water bath at 37 °C, 40 rpm for 30 min.
Downstream tips
- Store tubes at 4°C protected from light prior to analysing. Analyze on the flow cytometer within 1 hour.

Manufacturer protocol Publication protocol 1 Relevant paper

Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- This Assay was used for determining the percentage of S-phase cells

Upstream tips
- This Assay was used for determining the percentage of S-phase cells

Manufacturer protocol Publication protocol 1 Relevant paper

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