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|- Cells are fixed with 70% cold ethanol, and stored at −20 °C for 1 h
- Cells were washed with PBS containing 0.1% triton X-100 on a shaking water bath at 37 °C, 40 rpm for 30 min.
|- Store tubes at 4°C protected from light prior to analysing. Analyze on the flow cytometer within 1 hour.|