Cell cycle assay human - FaDu

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- Prior to the assay cells were either fixed in 70% ethanol and incubated with propidium iodide/Triton X-100 staining solution with RNase A (0.5ml PBS/BSA + 100U/ml RNase A + 0.5mg/ml propidium iodide

Upstream tips
- Prior to the assay cells were either fixed in 70% ethanol and incubated with propidium iodide/Triton X-100 staining solution with RNase A (0.5ml PBS/BSA + 100U/ml RNase A + 0.5mg/ml propidium iodide

Manufacturer protocol Publication protocol 1 Relevant paper

APC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells are harvested 72 h after transfection and continued with protocol.

Upstream tips
- Cells are harvested 72 h after transfection and continued with protocol.

Manufacturer protocol Publication protocol 1 Relevant paper

Cell Cycle and Apoptosis Analysis Kit

Beyotime

Upstream tips	Protocol tips	Downstream tips
	- Cells are fixed overnight at 4°C

Protocol tips
- Cells are fixed overnight at 4°C

Manufacturer protocol Publication protocol 1 Relevant paper

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