Cell cycle assay human - HaCaT

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Cell Cycle and Apoptosis Analysis Kit

Beyotime

Upstream tips	Protocol tips	Downstream tips
	- Cells are fixed at −20°C for 12 h after washing three times with cold PBS. - Cells were treated with 200 mg/mL RNase A and 50 μg/mL PI

Protocol tips
- Cells are fixed at −20°C for 12 h after washing three times with cold PBS. - Cells were treated with 200 mg/mL RNase A and 50 μg/mL PI

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Following seeing, cells were treated with PKF118-310, caperatic acid, physodic acid or camptothecin. - At −20 °C for at least 24 h cell are fixed with 70% ethanol.

Protocol tips
- Following seeing, cells were treated with PKF118-310, caperatic acid, physodic acid or camptothecin. - At −20 °C for at least 24 h cell are fixed with 70% ethanol.

Manufacturer protocol Publication protocol 1 Relevant paper

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Cells were fixed in 70% ethanol overnight. - To stain cells were resuspended in 500 µl of PI/RNASE staining solution.

Protocol tips
- Cells were fixed in 70% ethanol overnight. - To stain cells were resuspended in 500 µl of PI/RNASE staining solution.

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox