Cell cycle assay human - HCT-116

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- cells were fixed in ice-cold 70% ethanol at 4°C for >12h. - After staining cells were incubate 15 minutes instead of 10min.

Protocol tips
- cells were fixed in ice-cold 70% ethanol at 4°C for >12h. - After staining cells were incubate 15 minutes instead of 10min.

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
		When the cell count is less, ensure enough amount of cell were seeded at the start of the experiment

Downstream tips
When the cell count is less, ensure enough amount of cell were seeded at the start of the experiment

Manufacturer protocol Publication protocol 1 Relevant paper

FxCycle™ PI/RNase Staining Solution

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were fixed at 4 °C for 1 h and stored at −20 °C until ready

Protocol tips
- Cells were fixed at 4 °C for 1 h and stored at −20 °C until ready

Manufacturer protocol Publication protocol 1 Relevant paper

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