Cell cycle assay human - Hep G2

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells are treated with bicyclol for 24 h, or with 200 μl of bicyclol for 8,16 or 24 h before being fixed.

- Cells are fixed overnight at 4 °C
Upstream tips
- Cells were seeded andstarved overnight to achieve synchronization and then treated with 0–200 μM of H2O2 for 24 h.
Protocol tips
- Cells are fixed in cold 70% ethanol for 3 hours at −20°C.
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