No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
|- Cells were treated with different concentrations of 5‘-Cl (0.0 to 8.0 µM) for 24h or with 4.0 µM for various periods of time (0 to 48h).
- Cells were fixed in ice-cold 70% ethanol at -20°C for 3h.
- Stained with 200 µL of PI/RNAse reagent for 30 min.
|- Cells were treated with 10 µM of 11, or 1 µM of colchicine as a control, for 20 h..
-After incubation cells were centrifuged twice (before (1000 rpm for 5 min) and after (3000 rpm for 5 min) washing.
an appropriate amount of the antibody stock solution
1:50 with 1X BD Perm/Wash Buffer
- Store unused portions
of 1X BD Perm/Wash Buffer at 4°C.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product