Cell cycle assay human - HL-60

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells were treated with different concentrations of 5‘-Cl (0.0 to 8.0 µM) for 24h or with 4.0 µM for various periods of time (0 to 48h).

- Cells were fixed in ice-cold 70% ethanol at -20°C for 3h.

- Stained with 200 µL of PI/RNAse reagent for 30 min.
Protocol tips
- Cells were treated with 10 µM of 11, or 1 µM of colchicine as a control, for 20 h..

-After incubation cells were centrifuged twice (before (1000 rpm for 5 min) and after (3000 rpm for 5 min) washing.
APC BrdU Flow Kit

BD Biosciences

Upstream tips
- Dilute
an appropriate amount of the antibody stock solution
1:50 with 1X BD Perm/Wash Buffer

- Store unused portions
of 1X BD Perm/Wash Buffer at 4°C.
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