Cell cycle assay human - K562

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Upstream tips
- Cells (1×106 cells/well) were incubated with vehicle or 1-(3,4,5-trihydroxyphenyl)-dodecylbenzoate at their respectively IC50.
Protocol tips
- After 24 h of incubation cells were treated with the protocol
Upstream tips
- Post seeding, cells were treated with various doses of DOX or PTX with or without 5 μM of STFD for 48 hr.
Protocol tips
- Cells were fixed in 95% of ethanol overnight and stained
Downstream tips
- After 48 h of treatment, the effect of copanlisib on the cell cycle distribution in K562 cells was analyzed
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