Cell cycle assay human - MCF 10A

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells are fixed in cold 70% ethanol for 3 hours at −20°C.
Protocol tips
- Cells are labelled with EdU with 10 μM for 40 min
APC BrdU Flow Kit

BD Biosciences

Protocol tips
Incubate cells with antibodies for 15 minutes on
ice.
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