Cell cycle assay human - OVCAR-5

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

APC BrdU Flow Kit

BD Biosciences

Protocol tips
Cells are treated with 0.2 μM TPT, 0.5 μM PF477736, or both incubated for 16 hours.

Cells were incubated for 30min after adding BrdU.
Protocol tips
- Cells were stained and incubated for 45 min at 37 °C with 10 μg/ml of propidium iodide and 5 μg/ml of ribonuclease A in PBS.
Upstream tips
- Cells were analysed for cell cycle after 72h
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