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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were treated with DMSO, 10 μM NAC, or 10 μM pifithrin-β;
- cells were pre-exposed to 10 μM pifithrin-β for 3 h and then supplemented with 10 μM NAC.
- Cells are fixed with 70% ethanol at -20°C for overnight. |
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| Protocol tips |
- Cells were treated with DMSO, 10 μM NAC, or 10 μM pifithrin-β;
- cells were pre-exposed to 10 μM pifithrin-β for 3 h and then supplemented with 10 μM NAC.
- Cells are fixed with 70% ethanol at -20°C for overnight. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells weretransfected with an inhibitor for 48h. |
- Cells were stained for 20 min using Guava Cell Cycle Reagent. |
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| Upstream tips |
| - Cells weretransfected with an inhibitor for 48h. |
| Protocol tips |
| - Cells were stained for 20 min using Guava Cell Cycle Reagent. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - cells were adjusted to a concentration of 1×106 cells/ml |
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| Upstream tips |
| - cells were adjusted to a concentration of 1×106 cells/ml |
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