Cell cycle assay human - SH-SY5Y

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells were treated with DMSO, 10 μM NAC, or 10 μM pifithrin-β;

- cells were pre-exposed to 10 μM pifithrin-β for 3 h and then supplemented with 10 μM NAC.

- Cells are fixed with 70% ethanol at -20°C for overnight.
Upstream tips
- Cells weretransfected with an inhibitor for 48h.
Protocol tips
- Cells were stained for 20 min using Guava Cell Cycle Reagent.
Upstream tips
- cells were adjusted to a concentration of 1×106 cells/ml
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