Cell cycle assay human - U20S

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- The fixed cells were centrifuged at 800 Xg for 5 min at 4 °C - Suspended cells were treated with RNase A at 37 °C for 1 h followed by staining with Reagent.

Protocol tips
- The fixed cells were centrifuged at 800 Xg for 5 min at 4 °C - Suspended cells were treated with RNase A at 37 °C for 1 h followed by staining with Reagent.

Manufacturer protocol Publication protocol 1 Relevant paper

FxCycle™ Far Red Stain

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- cells were fixated with either 70% ethanol (Cyclin A, Aurora A and Plk1) or first 1% formaldehyde (1 h on ice) followed by 70% ethanol

Upstream tips
- cells were fixated with either 70% ethanol (Cyclin A, Aurora A and Plk1) or first 1% formaldehyde (1 h on ice) followed by 70% ethanol

Manufacturer protocol Publication protocol 1 Relevant paper

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- cells were fixed in 70% ethanol at −20°C for 4 h

Protocol tips
- cells were fixed in 70% ethanol at −20°C for 4 h

Manufacturer protocol Publication protocol 1 Relevant paper

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