Cell cycle assay human - U266

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

PI/RNASE Solution

Immunostep

Upstream tips	Protocol tips	Downstream tips
		- After cells were treated with PI/RNase staining solution (Immunostep),they were incubated for 20 minutes at RT

Downstream tips
- After cells were treated with PI/RNase staining solution (Immunostep),they were incubated for 20 minutes at RT

Manufacturer protocol Publication protocol 1 Relevant paper

APC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Cells were incubated for 1h with bromodeoxyuridine.

Protocol tips
- Cells were incubated for 1h with bromodeoxyuridine.

Manufacturer protocol Publication protocol 1 Relevant paper

FxCycle™ PI/RNase Staining Solution

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with PTC-209 (1 μM) for 24 h

Protocol tips
- Cells were treated with PTC-209 (1 μM) for 24 h

Manufacturer protocol Publication protocol 1 Relevant paper

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