Cell cycle assay mouse - C2C12

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Cells were fixed at −20 °C in 70% ice cold ethanol for at least 12–18 h and was done at different intervals.

Protocol tips
- Cells were fixed at −20 °C in 70% ice cold ethanol for at least 12–18 h and was done at different intervals.

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cells are seeded in DMEM + 10% FBS + 1% PS for 3 days. - Fixed overnight with 70% ethanol at −20 °C

Protocol tips
- Cells are seeded in DMEM + 10% FBS + 1% PS for 3 days. - Fixed overnight with 70% ethanol at −20 °C

Manufacturer protocol Publication protocol 1 Relevant paper

FxCycle™ PI/RNase Staining Solution

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
		- Do not wash the cells after staining with FxCycle PI/RNase Staining Solution

Downstream tips
- Do not wash the cells after staining with FxCycle PI/RNase Staining Solution

Manufacturer protocol Publication protocol 1 Relevant paper

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