Cell cycle assay mouse - L929

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Senescence Cells Histochemical Staining Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
The staining of senescent cells is pH dependent. Therefore, the cells cannot be incubated in a CO2 enriched atmosphere during the staining step	Stain cells with staining mixture (containing X-gal) at 37°C for 24 h

Upstream tips
The staining of senescent cells is pH dependent. Therefore, the cells cannot be incubated in a CO2 enriched atmosphere during the staining step
Protocol tips
Stain cells with staining mixture (containing X-gal) at 37°C for 24 h

Manufacturer protocol Publication protocol 1 Relevant paper

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Cells were fixed in 70% cold ethanol overnight at -20°C. - After staining, cells were Incubate on ice until measured.

Protocol tips
- Cells were fixed in 70% cold ethanol overnight at -20°C. - After staining, cells were Incubate on ice until measured.

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Washed cells with PBS by spinning at 4500 rpm.

Protocol tips
- Washed cells with PBS by spinning at 4500 rpm.

Manufacturer protocol Publication protocol 1 Relevant paper

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