Cell cycle assay mouse - L929

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Upstream tips
The staining of senescent cells is pH
dependent. Therefore, the cells cannot be
incubated in a CO2 enriched atmosphere during the
staining step
Protocol tips
Stain cells with staining mixture (containing X-gal) at 37°C for 24 h
Protocol tips
- Cells were fixed in 70% cold ethanol overnight at -20°C.

- After staining, cells were Incubate on ice until measured.
Protocol tips
- Washed cells with PBS by spinning at 4500 rpm.
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