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Found 3 matching solutions for this experiment
|- 3×103 cells/well in a 96-well plate were seeded for an assay.
|- After 24 h or culture, for another 2h cells were incubated with BrdU.
- anti-BrdU monoclonal antibody isincubated for 1 hr.
|- 10 μL CCK-8 reagent was added and the cells were incubated at 37 °C for 1-4h.
|- 2 × 104 cell per well were seeded for the assay.
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