No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 1 matching solution for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
|
Firstly, CD4+, CD8+, and CD56+ cells were labeled with the double-negative T cell biotin-antibody cocktail and anti-biotin MicroBeads. The magnetically labeled cells were separated using an LD Column on the autoMACS Separator. Secondly, the selected TCR (T cell receptor) α/β+CD4−CD8− cells present in the flow-through faction were incubated with Anti-PE MicroBeads and separated with MS column. |
The double-negative T cells were retained within the column and eluted by removing the column from the magnetic field. |
| Protocol tips |
| Firstly, CD4+, CD8+, and CD56+ cells were labeled with the double-negative T cell biotin-antibody cocktail and anti-biotin MicroBeads. The magnetically labeled cells were separated using an LD Column on the autoMACS Separator. Secondly, the selected TCR (T cell receptor) α/β+CD4−CD8− cells present in the flow-through faction were incubated with Anti-PE MicroBeads and separated with MS column. |
| Downstream tips |
| The double-negative T cells were retained within the column and eluted by removing the column from the magnetic field. |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!