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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Extract total genomic DNA was using the PureLink® Genomic DNA Mini Kit (K1820-02, Invitrogen) |
Follow the manufacturer’s instructions |
For data analysis: analyze the STR data using the DSMZ (German Collection of Microorganisms and Cell Cultures) online STR database |
| Upstream tips |
| Extract total genomic DNA was using the PureLink® Genomic DNA Mini Kit (K1820-02, Invitrogen) |
| Protocol tips |
| Follow the manufacturer’s instructions |
| Downstream tips |
| For data analysis: analyze the STR data using the DSMZ (German Collection of Microorganisms and Cell Cultures) online STR database |
| Upstream tips |
Protocol tips |
Downstream tips |
| Extract DNA from cell pellets using QiaAMP mini preps (Qiagen cat 51306) |
Use of a DNA amount of 0.15 ng, so that lower-level mixing of cell lines could be visible
Compare the STR profiles to known ATCC fingerprints (ATCC.org), to the Cell Line Integrated Molecular Authentication database
|
|
| Upstream tips |
| Extract DNA from cell pellets using QiaAMP mini preps (Qiagen cat 51306) |
| Protocol tips |
Use of a DNA amount of 0.15 ng, so that lower-level mixing of cell lines could be visible
Compare the STR profiles to known ATCC fingerprints (ATCC.org), to the Cell Line Integrated Molecular Authentication database
|
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