Cell migration / Invasion cell type - 4T1

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 1 matching solution for this experiment

Corning® BioCoat™ Matrigel® Invasion Chamber with 8.0 µm PET Membrane in four 6-well Plates

Corning

Upstream tips	Protocol tips	Downstream tips
Plate should be stored at -20C in the original packaging.	Incubate the Corning BioCoat™ Matrigel Invasion Chambers for 24 hours in a humidified tissue culture incubator, at 37C, 5% CO2 atmosphere. Scrubbing must be accomplished quickly to avoid drying of the cells adhering to the bottom surface of the membrane Stain cells with 0.05% crystal violet for 15 minutes

Upstream tips
Plate should be stored at -20C in the original packaging.
Protocol tips
Incubate the Corning BioCoat™ Matrigel Invasion Chambers for 24 hours in a humidified tissue culture incubator, at 37C, 5% CO2 atmosphere. Scrubbing must be accomplished quickly to avoid drying of the cells adhering to the bottom surface of the membrane Stain cells with 0.05% crystal violet for 15 minutes

Manufacturer protocol Publication protocol 1 Relevant paper

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