Cell migration / Invasion cell type - AGS

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 1 matching solution for this experiment

Cultrex BME Cell Invasion Assay, 96 well

R&D Systems

Upstream tips	Protocol tips	Downstream tips
Coat upper chamber with 0.5 X BME solution at 37°C in a CO2 incubator for 4 hours	Incubate bottom chamber at 37C in CO2 incubator for 24 hours

Upstream tips
Coat upper chamber with 0.5 X BME solution at 37°C in a CO2 incubator for 4 hours
Protocol tips
Incubate bottom chamber at 37C in CO2 incubator for 24 hours

Manufacturer protocol Publication protocol 1 Relevant paper

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