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|Add 300 µL of prepared cell suspension from step 3 to each insert.
Add 500 µL of serum free media in the presence or absence of chemo-attractant (e.g. 10% fetal bovine serum) to the lower chamber.
Cover plate and incubate for 24 hours at 37°C in a CO2 incubator (4-6% CO2)
|Measure the Optical Density at 560 nm.|