Cell migration / Invasion cell type - HEK293T

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 1 matching solution for this experiment

24-Well Haptotaxis Assays, Collagen I

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	Incubate for 4 hours in a cell culture incubator. Add 300μl of Lysis Buffer/CyQuantR GR dye solution

Protocol tips
Incubate for 4 hours in a cell culture incubator. Add 300μl of Lysis Buffer/CyQuantR GR dye solution

Manufacturer protocol Publication protocol 1 Relevant paper

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