Cell migration / Invasion cell type - HT-1080

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Protocol tips
Place chamber slides into a
5% CO2 tissue culture incubator at 37°C for 20 hours
Downstream tips
Increase concentration of blocking serum or protein in fluorescent staining buffer to decrease background if there is a poor staining
Protocol tips
Incubate in humidified atmosphere and 5% CO2 at 37°C for 4 hours
Protocol tips
Incubate for 19 hours at 37ºC in 5% CO2 atmosphere
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms