Cell migration / Invasion cell type - LoVo

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Upstream tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent).
Protocol tips
Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4- 6% CO2).
Protocol tips
Culture cells for 12 h in serum-free medium

Incubate 48 h for the migration assay and 72 h for the invasion assay
Protocol tips
Incubate 24 hours in a tissue culture incubator.
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