Cell migration / Invasion cell type - PC-3

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 5 matching solutions for this experiment

Upstream tips
Seed 0.5 × 10^6 to 1 × 10^6 cells
Protocol tips
Add 10% FBS complete medium in the lower chamber.

Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).
Downstream tips
Read plate at 570nm
ORIS™ Cell Migration Assay

Platypus Technologies

Protocol tips
Incubate cells in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.
Downstream tips
To read use 485/528 nm excitation/emission filters
Protocol tips
In the lower chnaber add medium supplemented with 10% FBS.

Incubate plates at 37 °C (5% v/v CO2) for 24 h,
Downstream tips
Read plate at 485 nm excitation, 520 nm emission
Protocol tips
In the lower chnaber add medium supplemented with 10% FBS.

Incubate plates at 37 °C (5% v/v CO2) for 24 h,
Downstream tips
Read plate at 485 nm excitation, 520 nm emission
Upstream tips
Suspend 100 000 cells/mL
Protocol tips
Incubate for 72 hours in a cell culture incubator.
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