Cell migration / Invasion cell type - SCC104

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 1 matching solution for this experiment

Protocol tips
Incubate at 37 oC in a CO2 incubator for 24 hours.

On the third day add 100 µl of Cell Dissociation Solution/ Calcein AM to bottom
chamber and incubate at 37 oC in a CO2 incubator for one hour
Downstream tips
Read plate at 485 nm excitation, 520 nm emission.
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