Cell migration / Invasion cell type - SCC2

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 1 matching solution for this experiment

CultreCoat Low BME Cell Invasion Assay, 96 well

R&D Systems

Upstream tips	Protocol tips	Downstream tips
	Incubate at 37 oC in a CO2 incubator for 24 hours. On the third day add 100 µl of Cell Dissociation Solution/ Calcein AM to bottom chamber and incubate at 37 oC in a CO2 incubator for one hour	Read plate at 485 nm excitation, 520 nm emission.

Protocol tips
Incubate at 37 oC in a CO2 incubator for 24 hours. On the third day add 100 µl of Cell Dissociation Solution/ Calcein AM to bottom chamber and incubate at 37 oC in a CO2 incubator for one hour
Downstream tips
Read plate at 485 nm excitation, 520 nm emission.

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox