Cell migration / Invasion cell type - Skov3

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	Add 10% fetal bovine serum) to the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).

Protocol tips
Add 10% fetal bovine serum) to the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).

Manufacturer protocol Publication protocol 1 Relevant paper

24-Well Cell Invasion Assays, Basement Membrane

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	Incubate for 24-48 hours at 37ºC in 5% CO2 atmosphere.	Measure the OD 560nm in a plate reader

Protocol tips
Incubate for 24-48 hours at 37ºC in 5% CO2 atmosphere.
Downstream tips
Measure the OD 560nm in a plate reader

Manufacturer protocol Publication protocol 1 Relevant paper

CultreCoat Medium BME Cell Invasion Assay, 96 well

R&D Systems

Upstream tips	Protocol tips	Downstream tips
Seed 2.5×10^4 cells	Incubate chamber at 37 oC in CO2 incubator for 24 hours

Upstream tips
Seed 2.5×10^4 cells
Protocol tips
Incubate chamber at 37 oC in CO2 incubator for 24 hours

Manufacturer protocol Publication protocol 1 Relevant paper

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