Cell migration / Invasion cell type - SW480

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Upstream tips
Seed 1×10^4 cells per well.
Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours (cell line dependent) to permit cell attachment
Upstream tips
Prepare a cell suspension containing 0.5-1.0 x 106 cells/ml in serum free media
Protocol tips
Incubate for 2-24 hours in a cell culture incubator.
Downstream tips
Read fluorescence with a fluorescence plate reader at 480 nm/520 nm.
Upstream tips
Add 1x10^5 endothelial cells
Protocol tips
Incubate 24 hrs in the cell incubator.
Downstream tips
Read plate at absorbance 540 – 570 nm
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