No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Seed 5 × 10^5 cells/transwell. |
- Reconstitute Matrigel basement membrane with 5 μg/cm^2 .
- Supplement with 1% FBS medium on both sides of the wells and stiluli on the lower side. |
|
| Upstream tips |
| - Seed 5 × 10^5 cells/transwell. |
| Protocol tips |
- Reconstitute Matrigel basement membrane with 5 μg/cm^2 .
- Supplement with 1% FBS medium on both sides of the wells and stiluli on the lower side. |
| Upstream tips |
Protocol tips |
Downstream tips |
| Seed 50,000 cells |
Incubate at 37oC in CO2 incubator for 24 hours |
Read assay chamber solutions (bottom) at 485 nm excitation, 520 nm emission |
| Upstream tips |
| Seed 50,000 cells |
| Protocol tips |
| Incubate at 37oC in CO2 incubator for 24 hours |
| Downstream tips |
| Read assay chamber solutions (bottom) at 485 nm excitation, 520 nm emission |
| Upstream tips |
Protocol tips |
Downstream tips |
| Seed 3 × 10^5 cells |
Incubate for 24hours at 37ºC in 5% CO2 atmosphere. |
Measure the OD 560nm in a
plate reader |
| Upstream tips |
| Seed 3 × 10^5 cells |
| Protocol tips |
| Incubate for 24hours at 37ºC in 5% CO2 atmosphere. |
| Downstream tips |
Measure the OD 560nm in a
plate reader |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!