Cell migration / Invasion cell type - T98G

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 2 matching solutions for this experiment

QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
Seed 2 × 10^5 cells	Incubate 24 hours in a tissue culture incubator.	Read OD at 560 nm

Upstream tips
Seed 2 × 10^5 cells
Protocol tips
Incubate 24 hours in a tissue culture incubator.
Downstream tips
Read OD at 560 nm

Manufacturer protocol Publication protocol 1 Relevant paper

24-Well Cell Invasion Assays, Basement Membrane

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
Seed 300,000 cells/well	Incubate for 48 hours at 37ºC in 5% CO2 atmosphere.

Upstream tips
Seed 300,000 cells/well
Protocol tips
Incubate for 48 hours at 37ºC in 5% CO2 atmosphere.

Manufacturer protocol Publication protocol 1 Relevant paper

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