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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| -It is highly critical that the chromatin is of appropriate size and concentration. |
-For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP.
-It is important to keep the tissue cold to avoid protein degradation.
-Use fresh formaldehyde that is not past the manufacturer's expiration date. |
-Once in solution, store 1M DTT at -20°C. |
| Upstream tips |
| -It is highly critical that the chromatin is of appropriate size and concentration. |
| Protocol tips |
-For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP.
-It is important to keep the tissue cold to avoid protein degradation.
-Use fresh formaldehyde that is not past the manufacturer's expiration date. |
| Downstream tips |
| -Once in solution, store 1M DTT at -20°C. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Cells were then treated using 10 μM rifampicin or 1‰ DMSO as control sample for 24 h. After the interval, the cells were harvested, and immunoprecipitation was conducted with the Imprint® Chromatin Immunoprecipitation Kit (Sigma‐Aldrich) according to the manufacturer's protocol. |
|
| Protocol tips |
| Cells were then treated using 10 μM rifampicin or 1‰ DMSO as control sample for 24 h. After the interval, the cells were harvested, and immunoprecipitation was conducted with the Imprint® Chromatin Immunoprecipitation Kit (Sigma‐Aldrich) according to the manufacturer's protocol. |
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