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Found 2 matching solutions for this experiment
|-Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here.
Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases.
|-Add protease inhibitors to all lysis solutions before use.
|-Use 2 µg for 25 µg of chromatin.
-Keep cells on ice between the rounds of homogenisations.
- Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.
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