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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| -Add protease inhibitors to all lysis solutions before use. |
-Use 4µg for 106 cells.
-Keep cells on ice between the rounds of homogenisations.
- Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.
|
|
| Upstream tips |
| -Add protease inhibitors to all lysis solutions before use. |
| Protocol tips |
-Use 4µg for 106 cells.
-Keep cells on ice between the rounds of homogenisations.
- Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.
|
| Upstream tips |
Protocol tips |
Downstream tips |
|
-5 - 10 µg per ChIP
-Avoid over fixation.
-purchase expensive, "hing end" sonicator to get reproducible shearing.
-Avoid repeated freeze/thaw cycles.
-Keep all reagents on ice when not in storage. |
|
| Protocol tips |
-5 - 10 µg per ChIP
-Avoid over fixation.
-purchase expensive, "hing end" sonicator to get reproducible shearing.
-Avoid repeated freeze/thaw cycles.
-Keep all reagents on ice when not in storage. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Chromatin was extracted from naive CD4+Foxp3− T cells polarized for 48–72 h under various conditions (1×106 cells) after fixation with formaldehyde. |
|
| Protocol tips |
| Chromatin was extracted from naive CD4+Foxp3− T cells polarized for 48–72 h under various conditions (1×106 cells) after fixation with formaldehyde. |
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