ChIP Anti-bodies H3K9me3

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

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Found 3 matching solutions for this experiment

Anti-trimethyl-Histone H3 (Lys9) Antibody

Millipore

Upstream tips	Protocol tips	Downstream tips
	Infected RBCs were crosslinked with 1 % formaldehyde (Catalog number—28908, THERMO Scientific) for 10 min, lysed and sonicated in sonication buffer (10 mM Tris–HCl pH 7.5, 200 mM NaCl, 1 % SDS, 4 % NP-40, 1 mM PMSF) to obtain an average chromatin size of 200–400 bp. Chromatin was pre-cleared using 50 µl of a 50 % protein A Sepharose (GE healthcare) slurry for 1 h at 4 °C with gentle inverting. Immunoprecipitations were carried out in 1 ml of IP buffer (20 mM Tris–HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1 % Triton-X 100). Three µg antibody was used per 20 µg purified chromatin. Input chromatin was obtained after preclearing by de-crosslinking and purified using the Qiaquick column (Qiagen) according to manufacturer’s instructions. Immunoprecipitations were carried out with inverting at 4 °C for 14–16 h.

Protocol tips
Infected RBCs were crosslinked with 1 % formaldehyde (Catalog number—28908, THERMO Scientific) for 10 min, lysed and sonicated in sonication buffer (10 mM Tris–HCl pH 7.5, 200 mM NaCl, 1 % SDS, 4 % NP-40, 1 mM PMSF) to obtain an average chromatin size of 200–400 bp. Chromatin was pre-cleared using 50 µl of a 50 % protein A Sepharose (GE healthcare) slurry for 1 h at 4 °C with gentle inverting. Immunoprecipitations were carried out in 1 ml of IP buffer (20 mM Tris–HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1 % Triton-X 100). Three µg antibody was used per 20 µg purified chromatin. Input chromatin was obtained after preclearing by de-crosslinking and purified using the Qiaquick column (Qiagen) according to manufacturer’s instructions. Immunoprecipitations were carried out with inverting at 4 °C for 14–16 h.

Protocol tips

Infected RBCs were crosslinked with 1 % formaldehyde (Catalog number—28908, THERMO Scientific) for 10 min, lysed and sonicated in sonication buffer (10 mM Tris–HCl pH 7.5, 200 mM NaCl, 1 % SDS, 4 % NP-40, 1 mM PMSF) to obtain an average chromatin size of 200–400 bp. Chromatin was pre-cleared using 50 µl of a 50 % protein A Sepharose (GE healthcare) slurry for 1 h at 4 °C with gentle inverting. Immunoprecipitations were carried out in 1 ml of IP buffer (20 mM Tris–HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 1 % Triton-X 100). Three µg antibody was used per 20 µg purified chromatin. Input chromatin was obtained after preclearing by de-crosslinking and purified using the Qiaquick column (Qiagen) according to manufacturer’s instructions. Immunoprecipitations were carried out with inverting at 4 °C for 14–16 h.

Manufacturer protocol Publication protocol 1 Relevant paper

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

Abcam

Upstream tips	Protocol tips	Downstream tips
-Add protease inhibitors to all lysis solutions before use.	-Use 2-4 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Upstream tips
-Add protease inhibitors to all lysis solutions before use.
Protocol tips
-Use 2-4 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.

Manufacturer protocol Publication protocol 1 Relevant paper

Histone H3K9me3 antibody (mAb)

Active Motif

Upstream tips	Protocol tips	Downstream tips
	-2 - 10 µg per ChIP. -Avoid over fixation. -purchase expensive, "hing end" sonicator to get reproducible shearing. -Avoid repeated freeze/thaw cycles. -Keep all reagents on ice when not in storage.

Protocol tips
-2 - 10 µg per ChIP. -Avoid over fixation. -purchase expensive, "hing end" sonicator to get reproducible shearing. -Avoid repeated freeze/thaw cycles. -Keep all reagents on ice when not in storage.

Manufacturer protocol Publication protocol 1 Relevant paper

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