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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-If any reagent has formed a precipitate, warm at 55 °C until dissolved. Allow to equilibrate to room temperature before use.
|
-Use 1 µg of antibody per well.
-The range of cells that can be used with this kit is 0.2–2.5 x 105/well/ChIP sample. Using more than 0.5 million cells per well will increase the non-specific binding and reduce the yield and specificity of the ChIP reaction. If higher yield of ChIP DNA is desired for downstream applications (e.g. ChIP-Seq., ChIP-chip): a) DNA could be pooled by eluting consecutively (with 50 µl of elution buffer) from multiple individual ChIP reactions. |
|
| Upstream tips |
-If any reagent has formed a precipitate, warm at 55 °C until dissolved. Allow to equilibrate to room temperature before use.
|
| Protocol tips |
-Use 1 µg of antibody per well.
-The range of cells that can be used with this kit is 0.2–2.5 x 105/well/ChIP sample. Using more than 0.5 million cells per well will increase the non-specific binding and reduce the yield and specificity of the ChIP reaction. If higher yield of ChIP DNA is desired for downstream applications (e.g. ChIP-Seq., ChIP-chip): a) DNA could be pooled by eluting consecutively (with 50 µl of elution buffer) from multiple individual ChIP reactions. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Keep away from light. |
-Avoid foaming or bubbles
when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-An optimal chromatin amount is 5-10 µg per reaction.
-Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use. |
|
| Upstream tips |
| -Keep away from light. |
| Protocol tips |
-Avoid foaming or bubbles
when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-An optimal chromatin amount is 5-10 µg per reaction.
-Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use. |
| Upstream tips |
Protocol tips |
Downstream tips |
- DO NOT cryo-preserve specimen with sucrose.
-Make fresh 1% formaldehyde before each experiment.
|
-Keep lysate ice-cold. Sonication produces heat, which can denature the chromatin. Allow time between cycles of
sonication to prevent sample overheating.
- DNA fragments should be in 200 -1000 bps in size.
-Add 1-10 µg of immunoprecipitating antibody. |
|
| Upstream tips |
- DO NOT cryo-preserve specimen with sucrose.
-Make fresh 1% formaldehyde before each experiment.
|
| Protocol tips |
-Keep lysate ice-cold. Sonication produces heat, which can denature the chromatin. Allow time between cycles of
sonication to prevent sample overheating.
- DNA fragments should be in 200 -1000 bps in size.
-Add 1-10 µg of immunoprecipitating antibody. |
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