CRISPR Hamster - Deletion CHO-K1 COSMC

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

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Upstream tips
The combination of a “plain” T7 promoter pET plasmid [i.e., pET-3a-d, pET-20b(+), etc.] with a T7lac
promoter plasmid is not recommended.
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