CRISPR Rat - Deletion PC12 myosin IIA (Myh9)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

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Found 1 matching solution for this experiment

MYH9 CRISPR/Cas9 KO Plasmid (h) + Lipofectamine™ 3000 Transfection Reagent

MYH9 CRISPR/Cas9 KO Plasmid (h)

Santa Cruz Biotechnology

Upstream tips
If transfecting more than one plasmid (i.e., CRISPR/Cas9 KO Plasmid with HDR Plasmid), mix Plasmid DNA at equivalent ratios.
Protocol tips
Do not add antibiotics to the Plasmid Transfection Medium
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