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Found 4 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
Cell suspension at a concentration of 10^5cells/ml was mixed in a 1:1 ratio withTrevigen LMAgarose (#4250-050-02) at 37◦C |
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The images of the comets can be analyzed using Cell Profiler software (version 2.1.1 rev 6c2d896). |
Upstream tips |
Cell suspension at a concentration of 10^5cells/ml was mixed in a 1:1 ratio withTrevigen LMAgarose (#4250-050-02) at 37◦C |
Downstream tips |
The images of the comets can be analyzed using Cell Profiler software (version 2.1.1 rev 6c2d896). |
Upstream tips |
Protocol tips |
Downstream tips |
Seed 1x10^5 cells/ml/well |
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Processed samples can be viewed and captured under the ZEISS® Axio Vert.A1 Microscope. Percentage DNA tail (%) and tail length of comets can be obtained using the OpenComet software . |
Upstream tips |
Seed 1x10^5 cells/ml/well |
Downstream tips |
Processed samples can be viewed and captured under the ZEISS® Axio Vert.A1 Microscope. Percentage DNA tail (%) and tail length of comets can be obtained using the OpenComet software . |
Upstream tips |
Protocol tips |
Downstream tips |
Seed 2 × 10^5 cells/m/well |
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Use TriTek Comet Image program to analyze the fluorescence images to circumscribe the “head” and the “tail” regions of each comet. |
Upstream tips |
Seed 2 × 10^5 cells/m/well |
Downstream tips |
Use TriTek Comet Image program to analyze the fluorescence images to circumscribe the “head” and the “tail” regions of each comet. |
Upstream tips |
Protocol tips |
Downstream tips |
Seed 2 × 10^5 cells/ml |
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Use TriTek Comet Image program to analyze the fluorescence images to circumscribe the “head” and the “tail” regions of each comet. |
Upstream tips |
Seed 2 × 10^5 cells/ml |
Downstream tips |
Use TriTek Comet Image program to analyze the fluorescence images to circumscribe the “head” and the “tail” regions of each comet. |
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