DNA gel extraction / PCR product purification Product size > 15Kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

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Found 7 matching solutions for this experiment

Upstream tips
The amount of agarose excised from the gel should
be as small as possible.
Protocol tips
Remove the flow-through by aspiration. Avoid
contamination of the collection tube rim.
Upstream tips
The amount of agarose
excised from the gel should
be as small as possible.
Protocol tips
Remove the flow-through
by aspiration. Avoid
contamination of the
collection tube rim.
Upstream tips
The amount of agarose
excised from the gel should
be as small as possible.
Protocol tips
Remove the flow-through
by aspiration. Avoid
contamination of the
collection tube rim.
Upstream tips
The amount of agarose
excised from the gel should
be as small as possible.
Protocol tips
Remove the flow-through
by aspiration. Avoid
contamination of the
collection tube rim.
Upstream tips
The amount of agarose
excised from the gel should
be as small as possible.
Protocol tips
Remove the flow-through
by aspiration. Avoid
contamination of the
collection tube rim.
ChargeSwitch PCR Clean-Up Kit

Thermo Fisher Scientific

Upstream tips
The amount of agarose
excised from the gel should
be as small as possible.
Protocol tips
Remove the flow-through
by aspiration. Avoid
contamination of the
collection tube rim.
Protocol tips
Follow manufacturer's instructions
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