DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

4 Matching solutions
2 Discussions

Found 2 discussions for this experiment

Discussion

5 years ago

5 years ago by Israel Lev Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

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Discussion

5 years ago

5 years ago by Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Found 4 matching solutions for this experiment

GeneJET Genomic DNA Purification Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Close the bag with GeneJET Genomic DNA Purification Columns tightly after each use	If residual solution is seen in the purification column after washing the column with Wash Buffer II, empty the collection tube and re-spin the column for an additional 1 min. at maximum speed (≥12000  g).

Protocol tips
Close the bag with GeneJET Genomic DNA Purification Columns tightly after each use
Downstream tips
If residual solution is seen in the purification column after washing the column with Wash Buffer II, empty the collection tube and re-spin the column for an additional 1 min. at maximum speed (≥12000  g).

Manufacturer protocol Publication protocol 1 Relevant paper

HiPurA™ Streptomyces DNA Purification Kit

HiMEDIA

Upstream tips	Protocol tips	Downstream tips
Examine the reagents for precipitation. If any kit reagent forms a precipitate (other than enzymes), warm at 55-65°C until the precipitate dissolves and allow cooling to room temperature (15-25°C) before use.

Upstream tips
Examine the reagents for precipitation. If any kit reagent forms a precipitate (other than enzymes), warm at 55-65°C until the precipitate dissolves and allow cooling to room temperature (15-25°C) before use.

Manufacturer protocol Publication protocol 1 Relevant paper

DNeasy Blood & Tissue Kit

Qiagen

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Manufacturer protocol Publication protocol 1 Relevant paper

Wizard® Genomic DNA Purification Kit

Promega

Upstream tips	Protocol tips	Downstream tips
	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Manufacturer protocol Publication protocol 1 Relevant paper

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