DNA isolation / purification Tissue - FFPE samples

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5 years ago

5 years ago by Nicolai Brandt Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Found 4 matching solutions for this experiment

FlexiGene DNA Kit (250)

Qiagen

Protocol tips
Use of 1hr incubation of samples of paraffin-embedded samples with FG2/Protease instead of 10 min (suggested by the manufacturer) to ensure complete tissue lysis
Manufacturer protocol Publication protocol 1 Relevant paper
EZ1 DNA Tissue Kit (48)

Qiagen

Protocol tips
Incubation of the samples with proteinase K for 1 h 30 min because tissue lysis is not complete after 1hr, which is suggested by the manufacturer
Manufacturer protocol Publication protocol 1 Relevant paper
GeneRead DNA FFPE Kit

Qiagen

Protocol tips
Follow manufacturer’s instructions
Manufacturer protocol Publication protocol 1 Relevant paper
AllPrep DNA/RNA FFPE Kit

Qiagen

Manufacturer protocol Publication protocol 1 Relevant paper
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