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Found 8 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
The range of the ladder is 200-2000 bp |
Thaw, mix and briefly centrifuge DNA ladder before use.
Prepare DNA ladder:
For E-Gel™ EX Agarose Gels, mix 5 µL of DNA ladder with 15 µL of water.
For E-Gel™ Agarose Gels, mix 10 µL of DNA ladder with 10 µL of water.
For E-Gel™ 48 Agarose Gels, mix 10 µL of DNA ladder with 5 µL of water.
The double-stranded DNA ladder can be visualized on 1-2% E-Gel agarose gels. |
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Upstream tips |
The range of the ladder is 200-2000 bp |
Protocol tips |
Thaw, mix and briefly centrifuge DNA ladder before use.
Prepare DNA ladder:
For E-Gel™ EX Agarose Gels, mix 5 µL of DNA ladder with 15 µL of water.
For E-Gel™ Agarose Gels, mix 10 µL of DNA ladder with 10 µL of water.
For E-Gel™ 48 Agarose Gels, mix 10 µL of DNA ladder with 5 µL of water.
The double-stranded DNA ladder can be visualized on 1-2% E-Gel agarose gels. |
Upstream tips |
Protocol tips |
Downstream tips |
The range of the ladder is 10 bp-300 bp |
Thaw, mix and briefly centrifuge DNA ladder before use.
Prepare DNA ladder:
For 4% E-Gel™ EX Agarose Gels, mix 4 µL of DNA ladder with 16 µL of water.
For 4% E-Gel™ Agarose Gels, mix and use the ladder without dilution.
For 4% E-Gel™ 48 Agarose Gels, mix 2 µL of DNA ladder with 13 µL of water |
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Upstream tips |
The range of the ladder is 10 bp-300 bp |
Protocol tips |
Thaw, mix and briefly centrifuge DNA ladder before use.
Prepare DNA ladder:
For 4% E-Gel™ EX Agarose Gels, mix 4 µL of DNA ladder with 16 µL of water.
For 4% E-Gel™ Agarose Gels, mix and use the ladder without dilution.
For 4% E-Gel™ 48 Agarose Gels, mix 2 µL of DNA ladder with 13 µL of water |
Upstream tips |
Protocol tips |
Downstream tips |
Size range: 10 bp to 700 bp |
For polyacrylamide gels, load 5 µl (0.5 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
For agarose gels, load 10 µl (1 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane |
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Upstream tips |
Size range: 10 bp to 700 bp |
Protocol tips |
For polyacrylamide gels, load 5 µl (0.5 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
For agarose gels, load 10 µl (1 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane |
Upstream tips |
Protocol tips |
Downstream tips |
Size range: 25 bp to 766 bp |
Distilled water (dH20)* or TE Buffer-4 μl
Gel Loading Dye, Purple (6X), no SDS-1 μl
DNA Ladder/Marker-1 μl
Total volume-6 μl |
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Upstream tips |
Size range: 25 bp to 766 bp |
Protocol tips |
Distilled water (dH20)* or TE Buffer-4 μl
Gel Loading Dye, Purple (6X), no SDS-1 μl
DNA Ladder/Marker-1 μl
Total volume-6 μl |
Upstream tips |
Protocol tips |
Downstream tips |
The range of the ladder is 50 bp to 1 kb |
Recommended Load: 5μl per lane
For optimum separation use 3% agarose gel |
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Upstream tips |
The range of the ladder is 50 bp to 1 kb |
Protocol tips |
Recommended Load: 5μl per lane
For optimum separation use 3% agarose gel |
Upstream tips |
Protocol tips |
Downstream tips |
Size range: 25bp to 766 bp |
Load 10 μl (0.5 μg) Quick-Load Purple Low Molecular Weight DNA Ladder per gel lane |
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Upstream tips |
Size range: 25bp to 766 bp |
Protocol tips |
Load 10 μl (0.5 μg) Quick-Load Purple Low Molecular Weight DNA Ladder per gel lane |
Upstream tips |
Protocol tips |
Downstream tips |
Low Range DNA Ladder consists of 11 DNA fragments ranging from 50 bp to 1 kb. |
Recommended Load: 5 μl per lane |
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Upstream tips |
Low Range DNA Ladder consists of 11 DNA fragments ranging from 50 bp to 1 kb. |
Protocol tips |
Recommended Load: 5 μl per lane |
Upstream tips |
Protocol tips |
Downstream tips |
The range of the ladder is 100bp to 3kb |
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Upstream tips |
The range of the ladder is 100bp to 3kb |
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