DNA Ladder Low Range

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

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Found 8 matching solutions for this experiment

Upstream tips
The range of the ladder is 200-2000 bp
Protocol tips
Thaw, mix and briefly centrifuge DNA ladder before use.
Prepare DNA ladder:
For E-Gel™ EX Agarose Gels, mix 5 µL of DNA ladder with 15 µL of water.
For E-Gel™ Agarose Gels, mix 10 µL of DNA ladder with 10 µL of water.
For E-Gel™ 48 Agarose Gels, mix 10 µL of DNA ladder with 5 µL of water.
The double-stranded DNA ladder can be visualized on 1-2% E-Gel agarose gels.
E-Gel™ Ultra Low Range DNA Ladder

Thermo Fisher Scientific

Upstream tips
The range of the ladder is 10 bp-300 bp
Protocol tips
Thaw, mix and briefly centrifuge DNA ladder before use.
Prepare DNA ladder:
For 4% E-Gel™ EX Agarose Gels, mix 4 µL of DNA ladder with 16 µL of water.
For 4% E-Gel™ Agarose Gels, mix and use the ladder without dilution.
For 4% E-Gel™ 48 Agarose Gels, mix 2 µL of DNA ladder with 13 µL of water
Upstream tips
Size range: 10 bp to 700 bp
Protocol tips
For polyacrylamide gels, load 5 µl (0.5 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
For agarose gels, load 10 µl (1 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane
Upstream tips
Size range: 25 bp to 766 bp
Protocol tips
Distilled water (dH20)* or TE Buffer-4 μl
Gel Loading Dye, Purple (6X), no SDS-1 μl
DNA Ladder/Marker-1 μl
Total volume-6 μl
Upstream tips
The range of the ladder is 50 bp to 1 kb
Protocol tips
Recommended Load: 5μl per lane
For optimum separation use 3% agarose gel
Upstream tips
Size range: 25bp to 766 bp
Protocol tips
Load 10 μl (0.5 μg) Quick-Load Purple Low Molecular Weight DNA Ladder per gel lane
Low Range DNA Ladder

Jena Bioscience

Upstream tips
Low Range DNA Ladder consists of 11 DNA fragments ranging from 50 bp to 1 kb.
Protocol tips
Recommended Load: 5 μl per lane
Upstream tips
The range of the ladder is 100bp to 3kb
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